Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Mar 6;19(6):1375–1389. doi: 10.1111/jcmm.12525

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

HCN4 expression in myocardial continuity between sinus venosus and posterior AV canal. (A) TNNI2 stain of HH19 chick embryo. Arrow indicates continuity between sinus venosus myocardium and posterior AV canal. (B) ISH shows HCN4mRNA expression in continuity (arrow). (C) Merge of ISL1, TNNI2, HCN4 and DAPI staining of E11.5 mouse embryo, showing the myocardial continuity between the sinus venosus and posterior AV canal. The ISL1+/HCN4+/TNNI2+ SAN (arrowhead) and RVV (arrow) are shown. Note the thick endocardial cushions (asterisks) in the AVC. (D–F) Higher magnifications of boxed area in C and D. ISL1+/TNNI2+/HCN4+ cells are seen in the continuity between sinus venosus myocardium and posterior AV canal myocardium. (E and F) Grey values of ISL1 (E) and HCN4 (F). AVC: atrioventricular canal; LA: left atrium; LB: long bud; LCV: left cardinal vein; LV: left ventricle; OFT: outflow tract; RA: right atrium; RCV: right cardinal vein; SV: sinus venosus; V: ventricle. (C–F) White: TNNI2, green: ISL1, red: HCN4, blue: DAPI; scale bars: 50 μm.