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. 2015 Mar 19;19(6):1418–1425. doi: 10.1111/jcmm.12576

Figure 4.

Figure 4

The effect of hyperglycaemia on MALAT1, IL-6 and TNF-α at later time-points. HUVECs incubated with 5 mM (NG) and 25 mM glucose (HG) showed (A) no significant alteration of MALAT1mRNA (measured by RT-PCR) expression after 24, 48 or 72 hrs. (B) IL-6 and TNF-α mRNA (measured by RT-PCR) and protein expressions (measured using ELISA) were increased following HG incubation at 48 hrs, but (C) returned to NG levels after 72 hrs of incubation [mRNA data are expressed as a ratio to 18sRNA and all data (mean ± S.E.) are normalized to controls, n = 3 or more per group, *: significantly different than control and P < 0.05, AU: arbitrary units of protein].