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. Author manuscript; available in PMC: 2015 Jun 8.
Published in final edited form as: J Neurochem. 2013 Nov 26;129(1):72–84. doi: 10.1111/jnc.12521

Figure 1. Disruption of the layers of the retina upon overexpression of AP-2δ.

Figure 1

(A) Protein lysates from E9 embryos in ovo electroporated with either GFP or GFP-AP-2δ RCAS expression constructs (two different eyes for GFP-AP-2δ) were separated in a 10% SDS-PAGE gel and transferred to a nitrocellulose membrane. AP-2δ was detected with rabbit anti-AP-2δ antibody. (B) Retinal tissue sections from E7, E10 and E13 embryos in ovo electroporated with GFP or GFP-AP-2δ RCAS expression constructs were stained with DAPI to label the nuclei. INBL, inner neuroblastic layer; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Photographs were taken with a 40X lens using a Zeiss LSM710 confocal microscope. Arrows indicate holes. Scale bar = 50 μm.