Fig. 5.

Coimmunoprecipitation of Kir6.2 with α-synuclein from mouse pancreas. Anti-α-synuclein antibody (BD610787) was used to immunoprecipitate α-synuclein from mouse pancreatic tissue extracts in a coimmunoprecipitation experiment. Immunocomplexes were characterized on Western blots using anti-Kir6.2 (sc-11228) and anti-α-synuclein (sc-7011-R). Equivalent aliquots of the initial input of each homogenate (Input) were analyzed. Homogenates in which secondary antibody only was used (SO-IP) served as a negative control. Both α-synuclein and Kir6.2 were coimmunoprecipitated using the anti-α-synuclein antibody (Syn IP).