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. Author manuscript; available in PMC: 2016 Feb 20.
Published in final edited form as: Oncogene. 2014 Dec 8;34(34):4519–4530. doi: 10.1038/onc.2014.386

Figure 1. Effect of CR infection on EZH2 expression and colocalization with proliferation marker.

Figure 1

A. Paraffin-embedded sections prepared from the distal colons of uninfected normal (N) or days 6-34 post-CR-infected mice (D6-D34) were stained with EZH2 antibody. Percentages represent percent cells positive for EZH2. Scale bar = 75 μm (n = 3 independent experiments). B. Paraffin-embedded sections prepared from uninfected normal (N) and either wild type CR or ΔescV mutant-infected mouse distal colons were co-stained with antibodies for EZH2 and PCNA (a marker of proliferation). DAPI was used to label nuclei. Scale bar = 100 μm (n = 3 independent experiments). C. Nuclear protein extracts prepared from the colonic crypts of mice described in B were subjected to Western blotting with antibodies specific to indicated proteins.