Fig 2. Comparison between the protein profile of the aqueous extracts and the corresponding aqueous and organic phases from the sponges C. nucula, T. aurantium and A. aerophoba and their effect on the release of MMPs from astrocytes.

A: Thirty μg of total proteins of the aqueous extract (AE) and aqueous phase (AP) and a corresponding equal volume of organic phase (OP) were subjected to SDS-PAGE on 10% polyacrilamide electophoretic gels. Molecular weight markers (sp) (BioRad) are indicated. B: Representative zymographic gels of supernatants from astrocytes activated with LPS (10 μg/ml) and simultaneously treated with the crude extracts from C. nucula (10 μg/ml), T. aurantium (10 μg/ml), A. aerophoba (10 μg/ml) or with equal amounts of the corresponding aqueous and the organic phases. Untreated and unstimulated cells represent negative control (CTRL).