Fig 5. Determination of vascular leakage in infected retina.

To determine the vascular permeability, infected eyes were embedded in OCT at 24h and the cryosections were subjected to IHC analysis. Fluorescent densities for CD31 (Green, biomarker for blood vessels) and Fibrinogen (Red, released from blood into the retinal tissue) were measured. Fluorescent intensities were scanned in three different regions of the retina in each slide and an average of 3 to 4 retina in each treatment group. Fold change was calculated by calculating the ratio between CD31 (Vascular marker) and Fibrinogen (Leakage marker) and presented as mean ± SD. Statistical significance was determined by One way ANOVA with Dunnett’s multi-comparison test. ***p<0.0005, **p<0.005, n = 6). Original magnification 20x.