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. 2015 Jun 8;10(6):e0129431. doi: 10.1371/journal.pone.0129431

Fig 4. Gene expression analysis in intra-donor unicellular BM CMP derived baso-E progeny.

Fig 4

(A) Intra-donor clonal cultures of baso-Es generated in the presence of AFT024 were assigned into groups based on low and high levels of γ-globin expression. qRT-PCRs for select genes were performed. Histograms represent the average fold differences in gene expression relative to GAPDH calculated as 2^(-ΔCt). Error bars represent the standard error of the mean between independent clones pooled into expression group. Statistical significance was determined using student’s t-test with significant values p<0.05 denoted. (B) Clonal cultures of baso-Es from two non-sickle donors (donor 1 and 2 from Fig 3) were co-cultured on AFT024 with or without 15μM HU. qRT-PCRs for select genes were performed on individual clones in triplicate. Fold differences relative to GAPDH calculated as above. Histograms represent the average expression of the pooled clones and error bars represent the standard error between individual clones in dose status group. Statistical significance determined as above.