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. 2015 Jun 8;10(6):e0129431. doi: 10.1371/journal.pone.0129431

Fig 5. Expression of candidate genes in clonal populations of baso-Es in the presence or absence of HU.

Fig 5

Clonal cultures of BM CMP derived baso-Es from two non-sickle donors (donor 1 and 2 from Fig 3) co-cultured on AFT024 with or without 15μM HU. qRT-PCRs for candidate genes were performed in triplicate. Fold differences relative to GAPDH were calculated as 2^(-ΔCt). Histograms represent the average expression of the pooled clones and error bars represent the standard error between individual clones in dose status group.