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. 2015 Jun 8;10(6):e0128290. doi: 10.1371/journal.pone.0128290

Table 1. Summary of GAL4 line embryonic expression patterns.

Gal 4 Driver Line Expression Time Expression Place Primary Expression Feature Embryonic SNS expression (stage) Larval Expression (mCD8 GFP)
Gsc A 46772 embryo 11–17 Surrounding brain lobes and weak midline (15–17) Exterior brain lobes - -
Gsc B 48376 embryo 11–17 Broad brain lobe expression (11–17); anterior sensory neurons (11–17); lining of esophagus (13–16) Full brain lobes - -
Gsc C 46773 embryo 13–17 Weak CNS expression (11–17) Weak CNS - -
Gsc D 48377 embryo 12–17 Strong CNS expression (12–17) Complete CNS - -
Gsc E 40381 embryo 11–17 Weak CNS expression (12–17) Weak CNS - -
Gsc F 40382 embryo 12–16 Esophagus and foregut/esophageal ganglion (12–16); mild outer brain lobe (12–16); small hindgut segment (12–16) SNS st 12–16 Esophagus (pharyngeal muscles?); hindgut
Gsc G 40383 embryo 11–17 Pre-migrating SNS (11); early esophagus and foregut (11–13); proventriculus/foregut (16/17); frontal ganglion and FNJ (17); posterior brain lobe cluster (15–17) Foregut/SNS st 11–17 -
Mun α 47237 embryo 10–17 Brain lobes (12–17); SNS precursors (10/11); anterior end of midline (12–16) Very early SNS st 11–14 Anterior midgut cell bodies; hindgut
Mun β 47238 embryo 11–17 Anterior tip of esophagus (13–15); esophageal ganglion (13); brain lobes (15–17); large posterior brain lobe cluster (16–17); anterior receptor cell clusters (11–17) Mid-stages SNS marker st 11–13 Anterior midgut cell bodies
Mun γ 47239 embryo 11–17 Lining developing esophagus (12–16); optic lobe precursors (11–12); weak brain lobe (15–17); receptor cells in anterior end of embryo (16–17) Early SNS/anterior sensory neurons st 11–15 -
Mun δ 47275 embryo 11, 15–17 SNS precursors (11); anterior sensory receptors (16–17) Mild anterior sensory neurons st 11 -
Mun ε 40663 embryo 13–17 Dorsal closure (13–17) Dorsal vessel - -
Mun Ζ 40664 embryo 11–17 Midline precursors (13–17); presumptive foregut/hindgut (9–11); lining of developing esophagus (13–15); developing brain lobes (13–17) Developing CNS st 13 Similar expression as Mun I
Mun Η 40665 embryo 13–17 Broad expression in small punctate 13–17 Nonspecific - -
Mun Θ 40666 embryo 11–17 Pre-migrating SNS and early esophagus (11); sporadic and nonspecific esophageal tissue (16–17); receptor cells in anterior end of embryo (13–17) Anterior dorsal sensory neurons st 11 None observed
Mun Ι 40667 embryo 11–17 Presumptive hindgut (11); spread along developing esophagus (13–17); weak midline glia expression (13–17); brain lobes (14–17) Early foregut/hindgut; midline glia st 15–17 Posterior to PV; anterior midgut cell bodies; midbrain?; IMR?
Fkh 1 47326 embryo 11–17 Hindgut lining (11–17); light expression in whole midgut (11–17) Hindgut lining - Hindgut
Fkh 2 48746 embryo 11–17 Weak CNS expression (11–17) Non specific - -
Fkh 3 48764 embryo 9–17 Foregut/hindgut (9–12); complete Intestinal tract (13–17); Malpighian tubules (13–16); mild CNS expression (13–17) CNS/Intestinal Anatomy outline - -
Fkh 4 48795 embryo 11–17 Similar to Fkh 3; intestinal tract expression (11–17); Malpighian tubules (15/16) Intestinal anatomy outline st 11–15 -
Twi 1 46150 embryo 13–17 SNS cluster (13); esophageal/pharyngeal muscles, dorsal side of esophagus/EG? (15–17) Esophageal/pharyngeal clusters st 13–17 Anterior sensory neurons
Twi 2 48725 embryo 13–17 Mild CNS expression (13–17) Weak CNS - -
Twi 3 48729 embryo 11–17 Developing anterior Sensory receptors (11–17); mild CNS (13–17); Anterior sensory neurons - -
Twi 4 48760 embryo 11–17 Developing CNS (11–17) CNS st 13,17 -
RetP1 (Herna#1 in pPTGAL) embryo 12–17 Distinct expression in cells of the dorsal fold (14); putative adult midgut precursors and other endoderm/endoderm adjacent cells (14–17); specific subset of brain cells (15–16); periventricular ganglion, dorsal pharyngeal muscles, Malpighian tubules (17) CNS; SNS; foregut/midgut/hindgut; brain lobes st 13–17 Brain; posterior to PV; anterior midgut cell bodies and hindgut
RetP2A (Herna#2 in pBPGUw) embryo 12–17 Pre-migrating SNS clusters (11–12); migrating SNS clusters (13–16); specific subset of brain cells, putative adult midgut precursors and other endoderm/endoderm adjacent cells and Malpighian tubules (15–17) CNS; SNS subset; foregut/midgut/hindgut; brain lobes st 12–17 SNS; posterior to PV; anterior midgut cell bodies
RetP2B (Herna#3 pBPGUw) embryo 12–17 Distinct expression in cells in the esophageal clusters (13–14); esophageal and periventricular ganglion, specific subset of brain cells, putative adult midgut precursors and other endoderm/endoderm adjacent cells (15–17) CNS; SNS subset; foregut/midgut/hindgut; brain lobes st 12–17 SNS; posterior to PV; anterior midgut cell bodies
RetPIA (Herna#4 pBPGUw) embryo 13–17 Tracheal/peripheral (ventral) expression (13–14); midgut lining (endoderm/endoderm adjacent cells) (14–17 and later) PNS (ventral); midgut - Midgut and hindgut
RetPIB (Herna#5 pBPGUw) embryo 13–17 Proventriculus (13–14); minimal midgut/hindgut lining (endoderm/endoderm adjacent cells) (15–17); cephalopharyngeal ganglia/pharyngeal muscles (17) Proventriculus; midgut; hindgut lining st 13–14 (Proventriculus) -
RetPIC (Herna#6 pBPGUw) embryo 12–17 (Anterior) midgut lining (endoderm/endoderm adjacent cells) (14–17); hindgut lining (16–17) Anterior midgut; hindgut - Midgut and hindgut
RetIA (Herna#7) pBPGUw) embryo 12–17 CNS, broad PNS expression, trachea (12–16); proventriculus (16–17) CNS/PNS; proventriculus st 16–17 (Proventriculus) -
RetIB (Herna#8 pBPGUw) embryo 11–17 Developing CNS (11); distinct expression in cells in the esophagus (12–17); proventriculus (16–17); (anterior) midgut lining (endoderm/endoderm adjacent cells) (15–17) Proventriculus; anterior midgut st 12–17 (Proventriculus) -