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. 2015 Jun 8;10(6):e0128887. doi: 10.1371/journal.pone.0128887

Fig 6. Squamous differentiation markers were also induced by suspension and confluent treatment.

Fig 6

The expression of keratin-1, keratin-13, keratin-4, TG-1 and involucrin was determined by real-time PCR for the FaDu (A); A-431(B) and HCC94 (C, F) cells after suspension or confluent culture. The protein level of keratin-13 was confirmed by Western blotting for the FaDu (D) and HCC94 (E) cells. S48h: suspension 48h. N: pre-suspension. C6: cells were cultured at confluence for six days. C6-R3P: cells were cultured at confluence for six days and then reseeded at sub-confluence for 3 cell passages. *, p<0.05, and **, p<0.01 versus the S100A7 experiential groups and the control groups. The results represent the average values from three independent experiments.