Table 1. Morphometric Cell Measurements.
| Tissue | Cortex | MPOA | ||||||
|---|---|---|---|---|---|---|---|---|
| Treatment | No Tx | Veh | T | P* | No Tx | Veh | T | P |
| Total Outgrowth (um) | 43.3±2.3 | 51.2±2.8 | 61.8±3.4 | <0.05 | 30.3±1.9 | 34.0±2.6 | 45.9±3.5 | 0.001 |
| Soma Size (um2) | 62.5±1.3 | 63.4±1.3 | 65.7±1.4 | ns | 60.3±1.0 | 63.0±1.2 | 68.3±1.3 | <0.01 |
| # of Processes | 2.8±.09 | 2.8±.08 | 3.0±.09 | ns | 2.4±.08 | 2.6±.09 | 2.8±.1 | ns |
| # of Branch Pts | 1.0±.11 | 1.2±.14 | 1.6±.16 | <0.05 | .68±.08 | .71±.10 | 1.1±.17 | ns(0.067) |
Data represent mean ± SEM obtained from 5 independent experiments using cells cultured for 3 days (DIV 3) that were derived from 5 separate GD53 male fetuses. Morphometric comparisons between untreated (No Tx), vehicle- (Veh) and testosterone (T)-treated (10 nM) cells were performed using the non-parametric Kruskal-Wallis one-way analysis of variance followed by the Dunn’s Multiple Comparison test. A level of P < 0.05 was considered to be statistically significant. No significant differences were observed between the No Tx and Veh groups.
*P values indicate measures that are significantly different between the Veh- and T-treated groups. ns = not significantly different (P>0.05).