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. 2015 Jun 8;10(6):e0129882. doi: 10.1371/journal.pone.0129882

Fig 1. Electrical stimulation and exogenous ATP induced increase of ROS production.

Fig 1

Muscle fibers were isolated, load with DCF (30min) and stimulated with electrical stimulation (ES) (20Hz) or exogenous ATP (10μM). A. Representative image in pseudo-color of a cell loaded with DCF in control condition (upper panel) and stimulated with ES (lower panel). B, representative traces of DCF fluorescence under control or stimulated with ES or exogenous ATP. C, muscle cells were stimulated with ES or ATP and the slope of fluorescence is shows (see material and method). D, muscle cells were stimulated with ES at 20Hz or 90Hz and the slope of fluorescence is show (see Materials and Methods) (n = 4, *p<0.05, **p<0.01).