Fig. 4. The effect of yacon extract on FBS-stimulated phosphorylation of ERK1/2 in C6 glioma cell.

(A) C6 glioma cells were seeded and then incubated in serum free media for 24 h. Cells were pre-treated with different concentrations of yacon (200 µg/mL and 400 µg/mL) for 1 h, and then stimulated with 10% FBS for 30 min. The expression levels of p-ERK 1/2 in cell lysates were examined by immunoblot analysis. (B) The graph shows the intensity of the bands relative to the untreated group. Results are presented as means ± SE, and are represent 3 independent experiments. Values with the same superscript letter are not significantly different, as determined by Tukey's multiple range test (P < 0.05).