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. 2015 Jun 8;10(6):e0128188. doi: 10.1371/journal.pone.0128188

Fig 2. SYBR Green melt profiling of SIV SGA reactions originated from blood plasma sample.

Fig 2

RNA was extracted from the blood plasma of an SIV infected macaque and used for cDNA synthesis. 24 SIV SGA PCR reactions were performed starting from the RT reaction using a working dilution of 1/33. (A) Agarose gel analysis of 24 SGA reactions. 1.2 μl of the 24 SGA reactions were loaded on 1.2% agarose gel. The positive reactions containing the 3.2-kb env amplicon (black arrow) are indicated with a red arrowhead. (B, C) SYBR Green melting analysis of the 24 SGA reactions. Melting reactions were performed using 1.2 μl of the SGA reactions shown in A and 2 μl of SYBR Green in a total volume of 8 μl. The graphs corresponding to the melting curves and the melting peaks are given in B and C respectively. The red curves are corresponding to the 5 positive reactions indicated in A.