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. 2015 Jun 8;209(5):653–670. doi: 10.1083/jcb.201412060

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© 2015 Rana et al.

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Figure 4. — STIM2β cannot activate Orai channels. (A) STIM2α but not STIM2β can reconstitute SOCE in Neuro2A cells when coexpressed with Orai1. (B–D) STIM2α-CAD, but not STIM2β-CAD, elevates basal [Ca²⁺]_i in Neuro2A cells cotransfected with Orai1 (B), Orai2 (C), or Orai3 (D). Expression levels of Orai1, 2, and 3 in B–D are not directly comparable (n ≥ 15 cells for each condition in all panels). Error bars show means ± SEM.