Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 8;209(5):739–758. doi: 10.1083/jcb.201411024

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Holtz et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 10. — Endogenous HHIP1 protein is secreted and accumulates in the epithelial BM in the embryonic lung. (A–D) Whole-mount images of E12.5 (A and B) and E14.5 (C and D) mouse lungs isolated from Hhip1^+/− (A and C) and Hhip1^−/− (B and D) embryos. (E–T) Immunofluorescent detection of β-Galactosidase (β-Gal; E, G–I, K, and L), HHIP1 (F–H, J–L, M, O–Q, S, and T), Laminin (N–P), E-Cadherin (ECAD; P), and HSPG2 (R–T) in sections isolated from E14.5 Hhip1^+/− (E–H and M–T) and Hhip1^−/− (I–L) lungs. (H, L, P, and T) DAPI staining reveals nuclei (blue). Arrows demonstrate overlap between HHIP1 and β-Gal (E–H) or HSPG2 (Q–T) protein expression in the lung mesenchyme. (E–H and M–T) Arrowheads highlight HHIP1 protein staining in the epithelial BM. A commercial HHIP1 antibody (R&D Systems) was used in E–P, whereas a newly developed HHIP1 antibody was used in Q–T. Bars: (A) 500 µm; (E) 50 µm.