Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 8;209(5):739–758. doi: 10.1083/jcb.201411024

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Holtz et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 8. — HS binding is required to localize HHIP1 to the neuroepithelial BM. (A–T) Immunofluorescent detection of HHIP1 (B, C, E, G, H, J, L, M, O, Q, R, and T) and Laminin (D, E, I, J, N, O, S, and T) in embryos electroporated with pCIG (A–E), mHhip1-pCIG (F–J), mHhip1^ΔHS1/2-pCIG (K–O), and mHhip1::CD4-pCIG (P–T) and isolated 24 hpe. (A, F, K, and P) DAPI stains nuclei (gray). (C, E, H, J, M, O, R, and T) Nuclear EGFP labels electroporated cells. (G–J) Note HHIP1 colocalization with Laminin in the neural tube (arrowheads) and surface ectoderm (arrows, insets). (U) Quantitation of HHIP1 fluorescent intensity normalized to GFP expression. A.U., arbitrary unit. (V) Data in U binned into signal measured within the BM or neural progenitors. Data are presented as mean ± SEM. P-values determined by Student’s two-tailed t test. Bars: (A) 50 µm; (G, H, I, and J, insets) 25 µm.