Table 1.
Minimum inhibitory concentration of the compound against Gram positive and Gram negative bacteria.
| Microorganism | Minimum inhibitory concentration (MIC) (μg/mL) | |
|---|---|---|
| Compound | Streptomycin sulfate | |
| Gram positive | ||
| Bacillus subtilis (MTCC 441) | 250 | 2.5 |
| Staphylococcus aureus (ATCC 25923) | 125 | 37.5 |
| Staphylococcus epidermidis (MTCC 3615) | 125 | 10 |
| Enterococcus faecalis (ATCC 29212) | 250 | 25 |
| Gram negative | ||
| Escherichia coli (ATCC 25922) | 62.5 | 25 |
| Klebsiella pneumoniae (ATCC 15380) | 125 | 25 |
| Pseudomonas aeruginosa (ATCC 27853) | 62.5 | 50 |
| Multi drug resistant | ||
| Staphylococcus aureus (MMC/3) | >10.00 | NT |
| Staphylococcus aureus (MMC/5) | >10.00 | NT |
| Staphylococcus aureus (MMC/6) | 10.00 | NT |
| Staphylococcus aureus (MMC/9) | 10.00 | NT |
| Staphylococcus aureus (MMC/16) | 10.00 | NT |
| Staphylococcus aureus (MMC/17) | 8.00 | NT |
| Staphylococcus aureus (MMC/18) | 8.00 | NT |
| Staphylococcus aureus (MMC/19) | 10.00 | NT |
| Staphylococcus aureus (MMC/25) | 10.00 | NT |
| Staphylococcus aureus (MMC/28) | 6.00 | NT |
| Staphylococcus aureus (MMC/34) | 8.00 | NT |
| Staphylococcus aureus (MMC/45) | 8.00 | NT |
| Staphylococcus aureus (MMC/47) | 8.00 | NT |
| Staphylococcus aureus (MMC/49) | 12.00 | NT |
MTCC: microbial type culture collection; ATCC: American type culture collection; MMC: strains from Madras Medical College; streptomycin: standard antibacterial agents; compound: isosteviol; NT: not test; for multi-drug resistant strains the concentrations of the compounds were tested in mg/mL level. Streptomycin did not show activity towards multi-drug resistant strains.