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. Author manuscript; available in PMC: 2015 Jun 9.
Published in final edited form as: Mol Cell Biol Res Commun. 2001 Nov;4(6):337–344. doi: 10.1006/mcbr.2001.0300

Figure 5. PMK proteins are stimulated by osmotic stresses in mammalian cells.

Figure 5

HEK-293 cells were transfected as indicated and incubated in medium containing either 0.5 M sorbitol or 0.7 M NaCl for fifteen minutes prior to harvest. Proteins were immunoprecipitated with a monoclonal anti-HA antibody and incubated with ATF-2 in kinase assays. Reactions were analyzed by SDS/PAGE and autoradiography.