Figure 4.

Maintenance of the Directive Axis in Nematostella

(A) Expression analysis of network components in morphant early planulae; lateral views, oral end is to the left. Scale bars represent 50 μm.

(B) Mathematical model correctly predicts expression patterns of all genes shown in (A) (color code as in ISH), as well as pSMAD1/5 localization (shown as heat maps) in the knockdowns. All expression patterns and pSMAD1/5 staining patterns are plotted onto mesogloea and shown as 2D projections of a 3D structure. Expression domains of NvDpp/NvBMP5-8/NvChd, although not identical, strongly overlap when plotted onto mesogloea. In the model they are controlled by the same mathematical expression and thus plotted together.

See also Figures S3 and S4.