Fig. 5.

TbCe1 knockdown results in accumulation of 5′-monophosphate RNA. Poly(A) RNAs from TbCe1 RNAi uninduced (−) and induced with tetracycline for the number of days indicated were subjected to ligation-mediated RT-PCR (Fig. S3). Splint-ligation products were detected by PCR using an anchor forward primer and oligo(dT) as a reverse primer (Top). Products were resolved on agarose gel. Control reactions in which the splint DNA was omitted were carried out in parallel. Internal control reaction was performed using SL-specific forward primer and an α-tubulin-specific reverse primer (Bottom). The positions of marker DNA (in kilobases) are indicated to the Right.