Fig. 2.

Sex-specific changes in hippocampal Bdnf gene expression and DNA methylation at P28 after prenatal BPA exposure. Gene expression analysis of (A) Bdnf, (B) Grin2b, and (C) Gadd45b in the P28 hippocampus of males and females prenatally exposed to BPA (200 µg/kg per day) or vehicle (CTL). Schematic representation of transcriptionally relevant CpG sites in Bdnf IV (D) and Grin2b (E) promoters in the vicinity of CREB-binding sites (purple box). BPA induced a sex-specific effect on DNA methylation of Bdnf IV CpG2 (F) that was primarily driven by hypermethylation induced in males (G). No CpG methylation changes were observed in the Grin2b promoter at P28 (H). *P < 0.05, **P < 0.01.