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Rolling activities of beads coated with wild-type HABD, the PD-state mutant, and the O-state mutant through the C-terminal anchoring. (A–D) Results of the detachment assays using beads coated with the PD-state mutant (A and B, open diamonds) and the O-state mutant (C and D, open squares), in comparison with those coated with the wild type (open circles). The percentages of beads remaining bound (A and C) and the mean velocities (B and D) were plotted at each shear stress.
