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. 2015 May 18;112(22):E2910–E2919. doi: 10.1073/pnas.1422264112

Fig. 5.

Fig. 5.

Nitroso-redox stress increases in CS cells. (A) Quantification of DCF fluorescence intensity per cell. Cells were treated with DCFDA to detect ROS levels. Representative immunofluorescence labelings are shown in Fig. S5A. (B–D) Quantitative RT-qPCR show mRNA expression levels of HIF1α (B); antioxidant factors catalase and SOD1, primarily mitochondrial antioxidant factors SOD2, GABPA/NRF2A, and peroxynitrite reductases PRDX2 and PRDX5 (C); and ROS-producing oxidases NOX1, XDH, DUOX1, DUOX2, and COX2, (y = log scales with the exception of COX2) (D). (E) Quantification of DHR123 fluorescence intensity per cell. Cells were treated with DHR123 to detect peroxynitrite levels. Representative immunofluorescence labelings are shown in Fig. S5D. Immunofluorescence, n = 30 cells from three independent experiments, mean ± SEM. RT-qPCR and immunoblots, n = 3 independent experiments, mean ± SD *P ≤ 0.05, ***P ≤ 0.001 versus 198VI, based on the unpaired t test.