Table 1.
Average equilibrium force, extension change, folding energy, folding energy barrier, and position and folding rate at the equilibrium force associated with folding of a single gp41 hairpin within the gp41 complex
| Gp41 complex | Equilibrium force, pN | Extension change, nm | Folding energy (kBT) | Transition state energy* (kBT) | Transition state position† (amino acids) | Rate at equilibrium force, s−1 |
| Gp41-ll | 8.9 (0.6) | 11.0 (0.6) | −23.5 (1.2) | 1.9 (1.6) | 26 (2) | 2.8 (1.6) |
| Gp41-ll-CΔ7 | 7.5 (0.7) | 7.8 (0.6) | −16.2 (1.0) | 2.1 (1.8) | 21 (1) | 22.8 (0.2) |
| Gp41-sl | 9.9 (0.8) | 10.2 (0.8) | −23.6 (1.3) | −1.2 (1.1) | 21 (1) | 12.7 (0.5) |
| Gp41-ll-hp2 (CHR2)ǂ | 9.7 (0.6) | 11.4 (1.0) | −24.1 (0.9) | NA | NA | 0.3 (0.1) |
| Gp41-ll-hp2 (CHR3) | 8.6 (0.5) | 13.2 (1.0) | −24.2 (2.0) | 3.4 (1) | 36 (2) | 0.2 (0.1) |
| Gp41-ll + DPC | 6.0 (0.6) | 6.5 (0.9) | −8.4 (3.2) | 10.2 (4.2) | 28 (6) | 0.5 (0.4) |
| Gp41-sl + DPC | 8.6 (0.9) | 8.3 (1.0) | −18.2 (4.2) | 6.2 (1.9) | 26 (2) | 1.3 (1.2) |
| Gp41-ll-ext + Triton | 8.9 (0.7) | 9.7 (1.2) | −22.0 (3.3) | 2.7 (2) | 38 (2) | 1.2 (0.1) |
| Gp41-ll-N554D | 8.1 (0.6) | 10.5 (0.9) | −20.3 (0.7) | 2.5 (2.2) | 25 (2) | 11.8 (1.8) |
The equilibrium force or rate is defined at an unfolding probability of 0.5. The SD of the average is shown in parentheses. NA, not assessed.
†
The number of amino acids from the C terminus of the CHR in the gp41 hairpin.
ǂThe transition of the second hairpin in this construct.