Table 1.

Summary of cancer mutations that are predicted to affect BS69 binding to H3.3K36me3

Cancer mutations	Major cancer association of each mutation
H3.3G34R/V	Cerebral hemisphere type pediatric HGA
H3.3K36M	Chondroblastoma
SETD2 mutation	Cerebral hemisphere HGA, ccRCC, ETP T-ALL

H3.3K36M and SETD2 deletion are reported to affect global H3K36me3 and therefore are expected to impair overall BS69 binding to chromatin. H3.3G34R/V, on the other hand, affects H3.3K36me3 only in cis and thus will alter BS69 binding only locally. The major cancer association of each mutation is indicated. HGA, high-grade astrocytomas; ccRCC, clear-cell renal carcinoma, ETP T-ALL, early T-cell precursor acute lymphoblastic leukemia.