Fig. 2.

OME rescues motility defects of LPS mutants. (A, Left) LPS mutants constructed in an A⁻S⁺ background exhibit S-motility defects as seen by reduced swarm expansion compared with the parental strain (DK1217). (Center) LPS mutants mixed with nonmotile (NM) donors, which were Tra⁺ and expressed WT LPS (DK6204), were stimulated to swarm. (Right) In contrast, an isogenic traA::km donor (DW1409) did not rescue and in the case of the wzm mixture produced a nonmotile sharp colony edge phenotype. Dashed lines represent the edge of the liquid inoculum after it dried. (B) Cells depleted for lpxC were defective for S-motility and were rescued when mixed with a nonmotile Tra⁺ donor but not when mixed with a traA::km donor. Strains are listed in Table S1. (Scale bar in A: 300 µm in A and B.)