Table 1.

Overview of the five MinION® runs

	Run1	Run2	Run3	Run4	Run5
DNA library	1	2	3	4	4
DNA fragment size	8 kb	20 kb	20 kb	20 kb	20 kb
Flowcell chemistry	R7	R7	R7.3	R7.3	R7.3
Number of reads	9,241	3,990	6,052	11,957	35,252
Cumulative size (Mb)	21.4	19.3	40.8	34.5	88.9
N50 size (bp)	5,388	11,288	10,217	12,729	13,967
Average size (bp)	2,314	4,830	6,746	2,886	2,523
% of 2D reads	6.5%	13.6%	43.3%	11.6%	9.7%
% of 2D bases	14.6%	27.1%	57.1%	42.7%	44.6%