Fig. 6.

Inhibition of MAFB gene expression suppresses cell migration, invasion and proliferation in vitro. a. RT-qPCR confirmed that the gene expression of MAFB was inhibited by MAFB specific siRNA. b. Western blotting shown the inhibition of MAFB gene expression on protein level through MAFB specific siRNA administration. c. The effect of MAFB-siRNA on CNE-2 migration. CNE-2 cells were cultured in 6-well plates. Images were captured at 0 h, 24 h, 48 h and 72 h respectively, after the wound was made. d. Effect of MAFB specific siRNA on CNE-2 cells migration through Membrane Transwell assay. e. Effect of MAFB specific siRNA on CNE-2 cell invasion by Matrigel invasion assay. f. MTT cell viability was assayed once per day from day 1 to day 6 after the transfection of MAFB specific siRNA or the negative control in CNE-2 cells. The experiments were performed at least 3 times. The data were showed as means ± SD. * indicates P < 0.05 compared with control group