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Expression and purification of rhμPlg. a, SDS-PAGE analysis of rhμPlg expressed by fed-batch fermentation. Lane 1, standard protein marker (14.4–116.0 kDa); Lane 1, uninduced P. pastoris; Lanes 2–9, P. pastoris induced with methanol for 5, 10, 15, 20, 25, 30, 35, and 40 h. b, M, standard protein marker. Lane 1, gel-filtration chromatography wash fraction; Lane 2, cation-exchange chromatography wash fraction 3; Lane 3, western blot of rhμPlg
