Figure 3. Results showing the effects of turning off the induced electric field on SCP2 cells on the “North” side of the coil after cells have been exposed to it for 8 hours. 8 hrs control (1):

Control experiments used for normalization, showing cells migrated in 0.1% serum media after 8 hours with no induced E field, as 100%. 8 hrs +E (2): Hindered migration (consistent with Fig. 2f) of SCP2 cells in the presence of an induced E field (8 hrs control (1) and 8 hrs +E (2): p < 0.001). 8 hrs +E 8 hrs −E (3): Migration of cells after 16 hours, with the induced E field for the first eight hours and the field shut off for the next 8 hours (8 hrs control (1) and 8 hrs +E 8 hrs −E (3): p < 0.001). 16 hrs control (4): Control experiments showing cell migration after 16 hours with no induced E field. Note that cell migration appears to be restored to normal levels despite hindered migration of SCP2 cells due to the induced E field for the first 8 hours (8 hrs +E 8 hrs −E (3) and 16 hrs control (4): p =0.342). 16 hrs +E (5): Migration of cells after 16 hours in the presence of an induced E field (16 hrs control (4) and 16 hrs +E (5): p < 0.001)