Fig. 4.

Emc inhibits the growth-suppressing activity of da over-expression. (A-D) Light microscope images of third instar eye-antennal discs containing flp-out clones of varying genotypes marked positively with GFP. Dorsal side is upwards and anterior is towards the right. The red arrow indicates the position of the morphogenetic furrow. All discs were photographed at 10× magnification. (A) Wild-type flp-out clones grow well in a wild-type background. (B) UAS-Da clones do not survive in the eye-antennal disc. (C) UAS-Emc flp-out clones are able to grow similar to wild-type clones. (D) Clones that overexpress both Da and Emc proliferate. (E) Chart depicting the average percentage of eye-antenna imaginal disc area occupied by overexpression clones of genotypes described in A-D. Statistical significance was calculated using Student's t-test and equal or unequal variance was determined using a F-test. The number of discs containing clones that were analyzed can be found in E. We screened 250 discs for Da-expressing clones and failed to recover a single such clone. Error bars represent s.d.