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(A) Sponge is tandem repeats of miRNA binding site after reporter gene driven by Pol II promoters. The imperfect paring between microRNA and sponge is diagrammed for miR-122. (B) Tough decoy RNAs (TuDs) contain two single-stranded miRNA binding sites, flanked by double-stranded stems that enhance stability and promote nuclear export. U6 promoter is used for high level expression of the miRNA inhibitors.
