Skip to main content
. Author manuscript; available in PMC: 2015 Jun 9.
Published in final edited form as: Semin Liver Dis. 2015 Jan 29;35(1):81–88. doi: 10.1055/s-0034-1397352

Figure 2.

Figure 2

Validation of miRNA toolkit in HEK293 cells. (A) miRNA sensor plasmid to monitor the miRNA activity. The strategy for construction of AAV plasmids expressing functional pri-miRNA fragments (B) and miRNA inhibitors, TuD RNAs (C). Cross validation of miRNA expression and inhibition plasmids (D). Top at (D) shows increasing amounts of a pri-miRNA producing vector inhibits expression of a miRNA sensor plasmid in HEK293 cells. The bottom shows the de-repression from anti-miR TuDs in a dose response manner when we fix the amount of pri-miR plasmids. ITR, inverted terminal repeat; ΔITR, mutated ITR; CB, chicken β-actin promoter with CMV enhancer; U6, U6 promoter; PA, poly (A); pre-miR, precursor miRNA; Fluc, Firefly luciferase; Rluc, Renilla luciferase; β-Gal, β-galactosidase; 3 × miR-xT, 3 miRNA perfect target sites.