Figure 3. In vitro dynamic effects of edaravone and diethyl maleate (DEM) treatments of human umbilical cord mesenchymal stem cells (hUCMSCs) on reactive oxygen species (ROS) production and GSH/GSSG ratio change after lipopolysaccharide (LPS)/H₂O₂ intoxication (n = 4).

(a) The production of ROS was stained by DCFH-DA of each group of hUCMSCs after different durations of LPS/H₂O₂ challenge. (b) Quantification of ROS production. (c) Cellular GSH/GSSG change of each group of hUCMSCs was examined by commercial kit. LPS, LPS/H₂O₂ challenge; Eda10, 10 μM edaravone; Eda20, 20 μM edaravone. Magnification 200x. “**” means P < 0.01 against control group; “***” means P < 0.001 against control group; “#” means P < 0.05 against LPS/H₂O₂ group; “##” means P < 0.01 against LPS/H₂O₂ group; “###” means P < 0.001 against LPS/H₂O₂ group.