FIGURE 5.

Exposure of primary spinal cord cultures to ACM-hSOD^G93A induces mitochondria morphological alterations. (A) Representative electron microscopy images of spinal cord cultures treated for 4 h with ACM-hSOD1^WT, ACM-hSOD1^G93A alone or ACM-hSOD1^G93A plus spermidine (1 μM). Scale bar, 0.1 μm. (B) Graph showing mitochondrial circularity of cells after treatment with the different ACMs with or without spermidine. (C) Graph showing percentage of total mitochondria size area of cultures under the same treatment shown in B, classified in four groups: “small” (S; < 0.2 μm²), “medium” (M; 0.21–0.4 μm²), “large” (L; 0.41–0.6 μm²) and “extra large” (XL; >0.6 μm²). Values represent mean ± S.E.M. from over 100 mitochondria per condition, analyzed by one-way ANOVA followed by a Tukey post hoc test. ^∗p < 0.05 relative to ACM-hSOD1^WT. ###p < 0.01 relative to ACM-hSOD^G93A without spermidine.