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. 2015 Jun 9;5:11158. doi: 10.1038/srep11158

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Copyright © 2015, Macmillan Publishers Limited

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(A, B) SHSY5Y, SKNBE2c, and SKNAS cells were silenced for HIF1A or EPAS1 expression (shHIF1A, shEPAS1, respectively) and treated with 5 μM (not shown) and 10 μM ATRA, or only with vehicle (V), for 6 days. Phenotypic changes were analyzed by counting the cells that showed the neuronal and flat-cell phenotypes, in six different fields for each experimental point. The data are expressed as percentages. Blue arrows, examples of neuronal cells; yellow arrows, examples of flat cells. (C) The mean neurite lengths of the counted cells (μm). (D) Gene expression analysis of TUJ-1, NEFL, GFAP and S100 performed using RT-PCR in the three NBL cell lines. Data are means of three experiments (* P ≤0.05; ** P ≤ 0.01).