Table 2. Production of mice mutated on the Abl locus using fCas9.
| Cas9 | gRNA | Trial | Injected/ transferred | No. of pre- and postnatal embryos (survival rate %a) | Mutant (%) | Monoallelic mutant (%) | Biallelic mutant (%) |
|---|---|---|---|---|---|---|---|
| fCas9 | A1+A2 | 1 | 71/54 | 18 (25.4) | 0 (0) | 0 | 0 (0) |
| 2 | 61/38 | 34 (55.7) | 0 (0) | 0 | 0 (0) | ||
| total | 132/92 | 52 (39.4) | 0 (0) | 0 | 0 (0) | ||
| D10A | A1+A2 | 1 | 64/54 | 20 (31.3) | 10 (50.0) | 10 (100) | 0 (0) |
| WT | A1 | 1 | 31/31 | 10 (32.3) | 8 (80.0) | 8 (100) | 0 (0) |
| A2 | 1 | 31/30 | 13 (43.3) | 6 (46.2) | 5 (92.3) | 1 (7.7) |
Numbers in parentheses represent the percentages calculated from the number of mutants relative to the number of embryos. FokI-dCas9 (fCas9), Cas9 D10A (D10A), Cas9 WT (WT).
aSurvival rates were calculated from number of embryos relative to number of injected zygotes.