Comparison of the levels of cell-cell fusion induced by wt gB and gBΔ867. BHK, COS, and Vero cells cotransfected with expression plasmids encoding wt gB, gD, gH, and gL (wtBDHL) or gBΔ867, gD, gH, and gL (BΔ867DHL) (40 ng of each in BHK and COS cells; 80 ng of each in Vero cells) plus the pcDNA 3.1(−) Myc-His/Lac vector (40 ng in BHK and COS cells; 240 ng in Vero cells). The amounts of DNA in all transfection mixtures were made equal by addition of pMTS1, as appropriate. Syncytium staining and quantification were performed as described in the legend to Fig. 2. The bars indicate SE.