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Immunostaining with MAb to gD of COS cells cotransfected with plasmids encoding gB, gD, gH, and gL alone (BDHL) (80 ng of each) (A and C) or gB, gD, gH, and gL plus pUL20-pcDNA and pgK (160 ng of each) (B and D). The amounts of DNA in all transfection mixtures were made equal by addition of pMTS1, as appropriate. Filled arrowheads indicate gD-labeled cell surfaces. Open arrowheads indicate a lack of gD on cell surfaces.
