FIG. 3.

(a) Incorporation of VP16-GFP into virions. Gradient-purified virions from wt or v41 infected cells were separated by SDS-PAGE, blotted, and probed with antibodies to VP16, GFP, or VP5 as indicated. VP16 was present in v41 virions as a 95-kDa protein and when corrected for VP5 loading was incorporated in similar amounts as native VP16 into wt virions. (b) Single-step growth curves for wt and v41 viruses. Vero cells were infected at an MOI of 10, and cells and media were harvested and titrated on Vero cells. Results are plotted as total PFU/ml for wt (squares) and v41 virus (triangles). The results indicate that the recombinant v41 replicates with similar efficiency to wt virus.