Figure 2.

Acute observations. Anesthesia-associated changes in acute behavioral responses after tail biopsy of mice. For all cohorts of mice, acute observation scores were recorded for the first 10 min after biopsy and for a second 10-min interval beginning at 60 min after biopsy. Mice that received a 5-mm biopsy had significantly (P ≤ 0.05) increased acute observation scores at 10 min. Neither isoflurane, Cetacaine, nor ethyl chloride significantly diminished acute responses. The horizontal bar denotes that all mice that received ethyl chloride (both biopsy and sham) had significantly increased acute observation scores at 10 min compared with control mice and mice that received isoflurane anesthesia (P = 0.01 for both). There were no significant differences in acute observation score related to biopsy or anesthetic treatment at 60 min after biopsy.