Skip to main content
. 2004 Aug;78(15):7945–7957. doi: 10.1128/JVI.78.15.7945-7957.2004

FIG. 5.

FIG. 5.

Interactions of TYMV 66K and 140K proteins in the YTHS. TYMV proteins (or protein segments) fused to the GAL4 BD or AD were used to transform yeast cells. Aliquots of transformed yeast cells and serial fivefold dilutions were dropped on both −LW and −LWH media. For some plasmid combinations (indicated by asterisks), 2.5 mM 3-amino-1,2,4-triazole was added to the medium to prevent self-activation of the HIS3 reporter gene. No growth (−) or growth (+) of yeast colonies on interaction-selective medium (−LWH) after 48 to 72 h of incubation at 30°C is indicated for each combination. Typical interactions are shown along with negative and positive controls (rows 9 and 10, respectively). For quantitative β-galactosidase assays for interactions in the YTHS, liquid cultures were grown under interaction-nonselective conditions at 30°C. β-Galactosidase assays were performed in triplicate sets with at least seven independent cultures for each strain, and the means (in Miller units) and standard deviations are indicated; nd, not determined.