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. 2004 Aug;78(15):7945–7957. doi: 10.1128/JVI.78.15.7945-7957.2004

FIG. 6.

FIG. 6.

Coimmunoprecipitation of 66K and 140K-derived proteins in TYMV replication complexes. TYMV replication complexes purified by glycerol gradient centrifugation (Fig. 3, fraction 13) were subjected to immunoprecipitation (IP) with anti-66K antiserum (lanes 2 and 7), the corresponding preimmune (P.I.) serum (lane 8), anti-140K antiserum (lanes 3 and 6), or the corresponding preimmune serum (lane 4). The immunoprecipitates and an aliquot of the replicase fraction corresponding to 40% of the input sample (lanes 1 and 5) were subjected to SDS-7% PAGE and immunoblot analysis with anti-66K (lanes 1 to 4) or anti-140K (lanes 5 to 8) polyclonal antibodies. The positions of molecular weight markers (Biolabs) (in thousands) and immunoglobulin (Ig) heavy chains are indicated.