TABLE 1.
Summary of two-hybrid interactions tested
| Vector encoding GAL BD fusion | Vector encoding GAL AD fusion | Interactiona |
|---|---|---|
| 2P-66K | pGadGH | − |
| pGad-140K(1-1259) | − | |
| pGad-140K(1-1044) | − | |
| pGad-140K(1-877) | − | |
| pGad-140K(1-555) | − | |
| pGad-140K(555-1259) | + | |
| pGad-140K(877-1259) | − | |
| pGad-140K(1044-1259) | − | |
| pGad-140K(555-1044) | + | |
| pGad-140K(622-1044) | + | |
| pGad-140K(687-1044) | + | |
| pGad-140K(748-1044) | ± | |
| pGad-140K(812-1044) | − | |
| pGad-140K(877-1044) | − | |
| pGad-140K(553-1044) | + | |
| pGad-140K(553-983) | + | |
| pGad-140K(553-968) | + | |
| pGad-140K(553-958) | + | |
| pGad-140K(553-949) | + | |
| pGad-140K(553-939) | + | |
| pGad-140K(553-930) | − | |
| pGad-140K(553-877) | − | |
| pGad-140K(687-983) | + | |
| pGad-140K(687-939) | + | |
| pGad-140K(731-939) | ± | |
| pGad-140K(748-939) | ± | |
| 2P-140K(555-1259) | pGadGH | − |
| pGad-66K(1-585) | + | |
| pGad-66K(1-511) | + | |
| pGad-66K(1-395) | + | |
| pGad-66K(1-382) | + | |
| pGad-66K(1-369) | + | |
| pGad-66K(1-356) | − | |
| pGad-66K(1-237) | − | |
| pGad-66K(1-202) | − | |
| pGad-66K(1-172) | − | |
| pGad-66K(1-78) | − | |
| pGad-66K(80-585) | − | |
| pGad-66K(172-585) | − | |
| pGad-66K(203-585) | − | |
| pGad-66K(237-585) | − | |
| pGad-66K(395-585) | − | |
| pGad-66K(17-395) | ± | |
| pGad-66K(33-395) | ± | |
| pGad-66K(49-395) | ± | |
| pGad-66K(65-395) | ± | |
| pGad-66K(81-395) | − | |
| pGad-66K(1-369) | + | |
| pGad-66K(49-369) | ± | |
| pGad-66K(65-369) | ± | |
| pGad-66K(81-369) | − | |
| 2P-140K(687-939) | pGadGH | − |
| pGad-66K(1-369) | + |
a
Interactions were scored as follows: −, no growth of colonies on −LWH selective medium after 48 to 72 h at 30°C; ±, growth of petite white colonies; +, growth of regularly sized pink colonies.