FIG. 3.

Editing of nonreplicating amber/W reporters in HEK293 cells. (A) Western blot analysis with wild-type reporters that express functional HDAg. (B) Western blot analysis with reporters that express RNA-binding-deficient HDAg. In this construct, the natural initiator codon has been mutated to alanine, and position 39 has been mutated to methionine. (C) RT-PCR analysis followed by StyI digestion of reporters that do not express HDAg. Lanes: 1, wild-type reporter; 2, 6×His reporter; 3, 6×His+compensatory reporter; 4, upstream reporter; 5, downstream reporter; 6, upstream-downstream combination reporter. The 479-nucleotide unedited RT-PCR product is indicated by a •, while the 393-nucleotide edited product is indicated by *. The percentage of editing was calculated as described in Materials and Methods. All abbreviations are as described in the legend to Fig. 2.