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Alignment of the nucleotide sequences of the 5′ UTRs of EV71 strains reveals six mutation hot spots. The first two isolates, Tainan/4643/98 (GenBank accession number AF304458) and Tainan/6356/98, are associated with encephalomyelitis, and the Tainan/5746/98 (accession number AF304457) strain is associated with HFMD. Only the areas with nucleotide variations are shown.
