Kinases are color coded for whether LC-MRM is able to detect ABPP peptides (gray), pY peptides (light brown), or both (dark brown). The inset (upper right) shows the total numbers of proteins included unique each assay and the overlap between ABPP and pY datasets. In total, 264 protein kinases are monitored with 412 ABPP peptides using 1,628 total transitions. For phosphotyrosine quantification, 126 proteins (including 60 kinases) are monitored with 377 pY peptides using 1,488 total transitions.