Skip to main content
. 2004 Aug;78(15):8159–8171. doi: 10.1128/JVI.78.15.8159-8171.2004

FIG. 2.

FIG. 2.

The Rluc-reporting replicon containing an HDVr at its 3′ end can be used to monitor viral translation and RNA replication. (A) Rluc-reporting replicon containing a 3′ HDVr (RlucRep-HDVr). Rluc reporter is fused in-frame with the open reading frame of the replicon in the position where the structural region was deleted. RlucRep-NS5mt-HDVr contains a single-nucleotide frameshift upstream of the active site of the RdRp domain of NS5. (B) Time course of the Rluc activity in BHK cells transfected with RlucRep-HDVr or RlucRep-NS5mt-HDVr. (C) IFA of BHK cells transfected with RlucRep-HDVr or RlucRep-NS5mt-HDVr at 72 h posttransfection.